Mouse Monoclonal Antibody to Pirh2
|
| Catalog# |
30005
|
| Clone# |
1H10
|
| Host and isotype |
Mouse IgG1
|
| Species reactivity |
Human; Rat
|
Lot# |
|
| Size |
0.1ml
|
Price |
$
280
|
| MW |
30kDa; 60kDa (homodimer)
|
Entrez Gene |
25898
|
| Aliases |
ARNIP; CHIMP; RNF199; RCHY1
|
| Description |
|
Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.
|
| Immunogen |
|
Purified recombinant fragment of human Pirh2 expressed in E. Coli.
|
| Applications |
Western Blot: 1: 500 - 1: 2,000
IF: 1: 200 - 1: 1,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.
|
| Formulation |
|
Ascitic fluid containing 0.03% sodium azide.
|
| Storage |
|
Store at 4℃, for long term storage, store at -20℃.
|
| Related product |
|
1. #20338, Mouse Monoclonal Antibody to p53.
|
| References |
1. Biochem Biophys Res Commun. 2007 Dec 14;364(2):344-50.
2. J Natl Cancer Inst. 2004 Nov 17;96(22):1718-21.
3. Exp Cell Res. 2006 Oct 15;312(17):3370-8.
4. Cell. 2003 Mar 21;112(6):779-91.
|
|
Figure 1: Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.
|
| |
|
Figure 2: Immunofluorescence analysis of Hela cells using Pirh2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
|
| |
|
| FOR RESEARCH USE ONLY |
