Mouse Monoclonal Antibody to BDH1
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| Catalog# |
30003
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| Clone# |
1A5
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human; Mouse
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Lot# |
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| Size |
0.1ml
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Price |
$
280
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| MW |
38kDa
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Entrez Gene |
622
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| Aliases |
BDH
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| Description |
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BDH1 (3-hydroxybutyrate dehydrogenase, type 1), it is a member of the short-chain dehydrogenase/reductase gene family. This protien forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. It catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism.
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| Immunogen |
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Purified recombinant fragment of human BDH1 expressed in E. Coli.
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| Applications |
Western Blot: 1: 500 - 1: 2,000
IHC (P/F): 1: 200 - 1: 1,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.
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| Formulation |
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Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4℃, for long term storage, store at -20℃.
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| Related product |
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| References |
1. Biochemistry. 1996 Jun 25;35(25):8158-65.
2. Biochemistry. 2000 Oct 3;39(39):11928-38.
3. Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15089-94.
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Figure 3: Immunohistochemical analysis of paraffin-embedded human liver cancer (left) and colorectal cancer tissues (right) using BDH1 mouse mAb with DAB staining.
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Figure 2: Western blot analysis using BDH1 mouse mAb against BDH1 (aa47-343)-hIgGFc transfected HEK293 cell lysate (1).
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Figure 1: Western blot analysis using BDH1 mouse mAb against HepG2 (1) and NIH/3T3 (2) cell lysate.
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| FOR RESEARCH USE ONLY |
