Mouse Monoclonal Antibody to MAP2K6
|
| Catalog# |
20402
|
| Clone# |
8E5
|
| Host and isotype |
Mouse IgG1
|
| Species reactivity |
Human
|
Lot# |
|
| Size |
0.1ml
|
Price |
$
280
|
| MW |
37kDa
|
Entrez Gene |
5608
|
| Aliases |
MEK6; MKK6; MAPKK6
|
| Description |
|
Mitogen-activated protein kinase kinase 6, also known as MAP2K6, it is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
|
| Immunogen |
|
Purified recombinant fragment of MAP2K6 expressed in E. Coli.
|
| Applications |
Western Blot: 1: 500 - 1: 2,000
IF: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.
|
| Formulation |
|
Ascitic fluid containing 0.03% sodium azide.
|
| Storage |
|
Store at 4℃, for long term storage, store at -20℃.
|
| Related product |
|
|
| References |
1. Mol Cell Biol. 2000 Jul;20(13):4543-52.
2. Biochem J. 1998 Dec 15;336 ( Pt 3):599-609.
3. J Biol Chem. 1998 Jan 16;273(3):1741-8.
|
|
Figure 1: Western blot analysis using anti-MAP2K6 monoclonal antibody against human recombinant MAP2K6 protein.
|
| |
|
Figure 2: Confocal immunofluorescence analysis of HepG2 (left) and PANC-1 (right) cells using anti-MAP2K6 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
|
| |
|
| FOR RESEARCH USE ONLY |
