Mouse Monoclonal Antibody to JAK3
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| Catalog# |
20400
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| Clone# |
5H2
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human; Mouse
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Lot# |
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| Size |
0.1ml
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Price |
$
280
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| MW |
125kDa
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Entrez Gene |
3718
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| Aliases |
JAKL; LJAK
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| Description |
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JAK3, Janus kinase 3. It is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease).
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| Immunogen |
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Purified recombinant fragment of human JAK3 expressed in E. Coli.
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| Applications |
Western Blot: 1: 500 - 1: 2,000
IF: 1: 200 - 1: 1,000
ELISA: Propose dilution 1: 10,000
Determining optimal working dilutions by titration test.
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| Formulation |
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Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4℃, for long term storage, store at -20℃.
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| Related product |
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| References |
1. J Biol Chem. 1995 Oct 20;270(42):25028-36.
2. Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6374-8.
3. Leuk Lymphoma. 2002 Dec;43(12):2355-62.
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Figure 2: Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using anti-JAK3 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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Figure 3: Confocal immunofluorescence analysis of 3T3-L1 cells using anti-JAK3 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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Figure 1: Western blot analysis using anti-JAK3 mAb against Jurkat cell lysate (1).
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| FOR RESEARCH USE ONLY |
