Mouse Monoclonal Antibody to PTEN
|
| Catalog# |
20399
|
| Clone# |
1B8
|
| Host and isotype |
Mouse IgG1
|
| Species reactivity |
Human; Mouse
|
Lot# |
|
| Size |
0.1ml
|
Price |
$
280
|
| MW |
54kDa
|
Entrez Gene |
5728
|
| Aliases |
BZS; MHAM; TEP1; PTEN1
|
| Description |
|
PTEN (phosphatase and tensin homolog) was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. This protein is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.
|
| Immunogen |
|
Purified recombinant fragment of PTEN expressed in E. Coli.
|
| Applications |
Western Blot: 1: 500 - 1: 2,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.
|
| Formulation |
Antibody are purified by protein G affinity chromatography.
Liquid in PBS containing 50% glycerol and 0.03% sodium azide.
|
| Storage |
|
Store at 4℃, for long term storage, store at -20℃.
|
| Related product |
|
|
| References |
1. Nat Genet. 1998 Aug;19(4):348-55.
2. Oncogene. 1999 May 20;18(20):3181-5.
3. Eur J Immunol. 2002 Apr;32(4):1196-204.
|
|
Figure 2: Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using anti-PTEN mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
|
| |
|
Figure 1: Western blot analysis using anti-PTEN monoclonal antibody against Hela (1) and NIH/3T3 (2) cell lysate.
|
| |
|
| FOR RESEARCH USE ONLY |
